Chromosome Research: Yeast Genetics and Imaging Data Analysis
This project investigates the molecular and physical mechanisms of meiotic homolog pairing in budding yeast. We integrate yeast genetics and live-cell imaging approaches to understand how chromosomes search for and align with their homologous partners during meiosis, a fundamental process in heredity.
We are recruiting undergraduate researchers for the Winter–Spring seasons. Students may apply for either or both positions. Preference will be given to students who can work during the winter season (except during holidays) and who have a strong interest in chromosomes and genetics.
1. Yeast Genetics Position
Students in this position will focus on how protein mutations affect meiotic homolog pairing and how meiotic homolog pairing occurs in polyploid cells.
Required Skills
- Basic molecular biology techniques (PCR, bacterial transformation, DNA extraction).
- Prior lab experience (including lab courses) is essential.
Application Materials
- Please describe your independent lab experience.
- If you have the experience of manual tetrad dissection in budding yeast, please highlight this experience.
- If you have not yet had the opportunity for prior lab work, explain your motivation for joining this position.
2. Imaging Data Analysis Position
Students in this position will analyze 4D live-cell imaging data of meiotic yeast cells. The goal is to track fluorescently labeled chromosome loci in 3D over time and quantitatively analyze their movement during meiosis.
Required Skills
- Experience with Python, R, MATLAB, Fiji (ImageJ), and/or other programming environments for image analysis (or other scientific studies).
Application Materials
- Applicants should share code samples (e.g., GitHub links) with brief explanations.
- If you have no prior coding experience, please describe why you are interested in this position.
Additional Application Request for both positions (optional)
If you are deeply interested in chromosome biology, please read the PI’s recent paper, Nozaki et al., 2024, and propose potential research questions related to chromosome organization and dynamics, homolog pairing mechanisms, evolution of chromosomes, and yeast genetics.