Developing Antifungal Resistance in A. fumigatus Strain

The goal of this project is to uncover the genetic underpinnings of pathogenic fungus Aspergillus fumigatus growing resistance to Amphotericin B, a key antifungal drug used in clinical settings. Using experimental evolution, we aim to identify the genetic changes that allow A. fumigatus to survive and adapt under drug stress. The project will focus primarily on the well-characterized A. fumigatus strain CEA10. The student researcher will gain hands-on experience with a range of microbiology and molecular biology techniques including sterile culture work, inoculation, DNA extraction, conidial quantification, and preparation of media with antifungal compounds. As the project progresses, there may also be opportunities to assist with genomic analysis comparing experimentally evolved and naturally resistant strains. Students with prior laboratory experience are encouraged to apply, but no previous training is required, only curiosity and a willingness to learn. All necessary instruction and aid will be provided. This is an excellent opportunity for a student interested in microbiology, genetics, or drug resistance who wants to be directly involved in discovery-driven research.