Image analysis and quantification of glial cell development in mice

Our lab studies glial cells that the myelinate the axons of neurons, which facilitates information transmission across brain circuits. The focus of this project is to delineate stages of cellular development using confocal images of brain tissue samples from transgenic reporter mice. We are looking for students who are interested in cell biology and the focus of this project is to help improving strategies for image processing and quantification to better understand these cells. Students will use image analysis software (e.g. Nikon NIS-Elements software, ImageJ) and process data for visualization and statistical analyses using R programming language and Microsoft Excel. 

In a brief essay, please answer the following questions: After reading some of our papers, what do you see as the most compelling part of our research? What questions remain unanswered?

 

Name of research group, project, or lab
Richardson Lab
Why participate in this opportunity?

Students who participate in this opportunity will develop a variety of skills for anatomical, cellular, and molecular understanding of the developing brain across the lifespan and the impact alcohol use has on the oligodendrocyte cell lineage. Once trained, image analysis software allows for remote work and opportunities for presenting at local conferences.

Representative publication or further information
Logistics Information:
Subject Category
Cellular Biology
Neuroscience
Student ranks applicable
Sophomore
Junior
Senior
Student qualifications

Students who have taken Cellular Biology courses will have greater success in this project. Minimum requirements include understanding and use of Microsoft Office or similar software for data management, writing, and presentations. Students with knowledge in statistical analyses and R-language are encouraged to apply. 

Time commitment
8-10 h/wk
Position Types and Compensation
Research - Independent Study or Research Assistant credit
Number of openings
4
Techniques learned

Techniques learned in this project include:

  • Identifying anatomical regions of the mouse brain
  • Image analysis in 2D and 3D using NIS-Advanced Research Software
  • Developing custom cell counting program in General Analysis 3
  • Data management and handling in R and Microsoft excel
  • Data visualization in R and Microsoft excel
  • Statistical analyses of data in R and Microsoft excel
Project start
Fall 2025
Contact Information:
Mentors
afloresbonil@umass.edu
Graduate Student
margotschmit@umass.edu
Name of project director or principal investigator
Heather Richardson
Email address of project director or principal investigator
hrichardson@cns.umass.edu
4 sp. | 7 appl.
Hours
8-10 h/wk
Project categories
Neuroscience (+1)
Cellular BiologyNeuroscience