Research about how sea slugs process smells

This project investigates the organization of a sea slug brain using modern molecular and computational tools, focusing on the sense of smell (olfaction). The goal is to understand how the rhinophore (the slug equivalent of a nose) works in the nudibranch, Berghia stephanieae. We will employ a combination bioinformatic approaches and molecular techniques including single-cell RNA sequencing to:  identify, describe, and classify the genes that code for receptor proteins involved in olfaction and then localize their expression to neurons.

It is often assumed that processing the multitude of signals that an animal receives from its environment happens in the brain (or central nervous system, CNS) rather than in the nerves outside the brain (peripheral nervous system, PNS). However, we have evidence that suggests that sea slugs begin to process olfactory information in the PNS of their rhinophores. Understanding whether the PNS in molluscs performs functions that we usually think are reserved for the CNS could change how we think about what the brain actually does.

Ideally, you will be assisting us with all stages of the project. Training will be provided. You should be comfortable working both at the bench in the lab and with computers. Some of the protocols involved demand careful attention to detail over long periods of time. 

Please indicate in your essay: i) why are you interested in this opportunity and what stands out to you the most; ii) examples of projects or tasks that you have been involved with, which required a long-term commitment and attention to detail; iii) a brief description of how this opportunity would assist you in your career goals.

Name of research group, project, or lab
Katz lab
Why participate in this opportunity?

This project will provide you with an opportunity to be involved in cutting-edge neuroscience research, gaining a deeper understanding of how this type research is conducted. It will also help you learn about and get hands-on experience using modern methodologies at the forefront of biological/biomedical research (bioinformatics and/or single-cell RNA sequencing) and develop computational and/or benchwork skills that will be advantageous at future stages of your scientific career.

You will be part of the fun-loving Katz Lab, which also includes three friendly and hard-working postdocs, graduate students, and multiple several other undergrads. You will be supervised by Dr. Jorge Pérez-Moreno. You will be expected to attend a weekly lab meeting and present your work in lab meeting and as a written semester report.

Representative publication or further information
Logistics Information:
Subject Category
Neuroscience
Student ranks applicable
Sophomore
Junior
Student qualifications

The project is best suited for a patient and perseverant person with a drive for learning new things.
Basic knowledge of cell biology & genetics is recommended; we will help you get up to speed if necessary. 
Comfort in a lab environment and attention to detail is a must. We will train you, but you will eventually need to be comfortable to work semi-independently.

Coding experience is helpful but NOT required, although familiarity with computational work (of any kind) is recommended as you will also be learning & assisting with bioinformatics work. 

Time commitment
8-10 h/wk
11-14 h/wk
Position Types and Compensation
Research - Ind. Study Credit
Research - Paid, Work-Study Req.
Number of openings
2
Techniques learned

Bioinformatics (data-mining, sequence manipulation, phylogenetics, transcriptomics/RNAseq analyses)

Single-cell RNA sequencing (Cell dissociation, RNA isolation, quantification, gel electrophoresis, library preparation).

In situ hybridization chain reaction.  

Laser scanning confocal fluorescence microscopy.

Contact Information:
Mentors
pkatz@umass.edu
Faculty
jperezmoreno@umass.edu
Postdoctoral Research Associate
Name of project director or principal investigator
Paul Katz
Email address of project director or principal investigator
pkatz@umass.edu
2 sp. | 29 appl.
Hours
8-10 h/wk (+1)
8-10 h/wk11-14 h/wk
Project categories
Neuroscience