Wastewater Bioreactors using Spent Mushroom Substrate

I am using a waste product from the mushroom farming industry, spent mushroom substrate, to culture and grow microbial communities that clean domestic wastewater. To perform these experiments, it involves frequent trips to the Amherst Wastewater Treatment Facility to collect wastewater, maintaining and operating bioreactors, learning some microbial culturing and molecular techniques, and performing some large dataset analysis in RStudio. Because of the multifaceted nature of this, you can discuss with me best ways to engage with this project to suit your learning goals from 1) engineering- maintaining reactors and running smaller microbial kinetics experiments 2) microbiology- culturing, DNA extraction, qPCR, and some fluorescent microscopy work and 3) bioinformatics- learning how to use RStudio to perform statistical analysis of a dataset (this won't be until the end of the semester).

Name of research group, project, or lab
Dr. Caitlyn Butler's lab in Engineering Lab 2
Why participate in this opportunity?

This opportunity is a really fun multifaced project that connects a lot of interdisciplinary interests from engineering, sustainability, and microbiology. If you're interested in at least 2 of those, this would be a really cool way to develop your skills and expertise while branching out a little. Additionally, you will have the opportunity to collaborate on a research publication and I'll help train you in the next steps to become a graduate researcher.

Logistics Information:
Field(s) of Study
Civil Engineering
Environmental Science
Microbiology
Student ranks applicable
Sophomore
Junior
Senior
Open to Honors Thesis Work
Student qualifications

I'll work with anyone who has interest and a great mindset.

If you've got some DIY experience or problem solving skills- those will go a long way!

Physically- To maintain the bioreactors, you should be able to comfortably lift 50lbs (~40lbs is a 5 gallon bucket). These are quite heavy, and we use a golf cart, and other carts to transit them between the wastewater treatment facility and our laboratory- but you will need to assist with loading and unloading. We can do these trips together and coordinate with others in the lab.

 

Time commitment
1-4 h/wk
5-7 h/wk
8-10 h/wk
Position Types and Compensation
Credit for Research and Teaching
Number of openings
2
Techniques learned

Bioreactor maintenance

Microbial kinetics / Chemical analysis (ammonia, nitrate, carbon, phosphorous)

Fluorescent microscopy

Statistics

DNA extraction

qPCR

 

 

 

 

Project start
January 2027
Contact Information:
Mentor
julietjohnst@umass.edu
Research Fellow
Name of project director or principal investigator
Dr Juliet Johnston
Email address of project director or principal investigator
julietjohnst@umass.edu
2 sp. | 0 appl.
Time commitment
1-4 h/wk (+2)
1-4 h/wk5-7 h/wk8-10 h/wk
Field(s) of Study
Environmental Science (+2)
Civil EngineeringEnvironmental ScienceMicrobiology