Zebrafish Feeding and Maintaining Transgenic Lines

The Karlstrom Lab investigates the formation and growth vertebrate brain using zebrafish, with a focus on the regulation of neural stem cells.  We are  looking for a work/study eligible student to help with the feeding and maintenance of the zebrafish facility through the winter break that could continue into the Spring semester and next summer.   Feeding and maintenance require about 2 hours of work daily, and we are  looking for someone who could take on several daily feedings over the holidays and through January, including some weekend feedings on a rotating basis.  This position could grow into an entry-level independent study project in the Spring that could include genetic crossing, PCR-based genotyping, and using fluorescence microscopy to identify transgenic fish. The student will start by learning zebrafish husbandry and breeding techniques, then progress into learning the molecular genotyping techniques (PCR genotyping) and the microscopy skills needed to identify fish that carry transgenes.  
This position is for a work/study student who would be paid for fish feeding duties. 

Name of research group, project, or lab
Karlstrom Lab
Why participate in this opportunity?

This project would be great for students who will be in Amherst over the Winter break and are interested in learning about zebrafish as a research model system, and/or in examining the mechanisms that regulate neural stem cells and brain growth.

Logistics Information:
Field(s) of Study
Developmental Biology
Neuroscience
Student ranks applicable
Freshman
Sophomore
Junior
Senior
Student qualifications

We are looking for someone who is reliable (the fish will depend on you), has good attention to detail, is curious to learn, and who has the ability to trouble-shoot problems.  We need someone who will be in Amherst over the winter break and potentially could work next summer as well.

Time commitment
1-4 h/wk
5-7 h/wk
8-10 h/wk
Position Types and Compensation
Credit for Research and Teaching
Paid - Work-Study Required
Unpaid - Volunteer
Number of openings
1
Techniques learned
  • Fish husbandry: feeding ~2 hours twice a week, occasional weekend shifts.
  • Fish Breeding: setting up fish for spawning, collecting eggs, sorting fertilized eggs.
  • Microscopy: identifying fluorescent transgenic fish and learning stages of embryogenesis
  • PCR based genotyping
Project start
November/December 2026
Contact Information:
Mentor
rolfk@umass.edu
Professor/ Principal Investigator
Name of project director or principal investigator
Rolf Karlstrom
Email address of project director or principal investigator
rolkf@umass.edu
1 sp. | 0 appl.
Time commitment
1-4 h/wk (+2)
1-4 h/wk5-7 h/wk8-10 h/wk
Field(s) of Study
Developmental Biology (+1)
Developmental BiologyNeuroscience